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Methodology for co-cryostorage of virus isolates in their host plants and relevant data
Bilavčík, A. ; Bjerkestrand, R. ; Bjelland, B. ; Blystad, D.-R. ; Bobrova, O. ; Fránová, Jana ; Faltus, M. ; Hamborg, Z. ; Hammond Hammond, S. D. ; Koloniuk, Igor ; Zámečník, J.
Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored.
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Methodology to identify missing genetic resources in culture collections, strategy to fill the gaps
Komínek, Petr a kolektiv
The methodology was created under the National programme on conservation of microbial genetic resources of economical imortance. Its preparation was planned in the beginning of a current period of solving the National programme (2018-2022). The methodology describe procedures for the evaluation of specialization of individual collections from the point of view variability of genetic resources held on the collections. The goal of the methodology is to improve the work of microbial collections for the sustainable conservation of microbial genetic resources.
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On farm conservation of obsolete cultivars and landraces methodology
Holubec, Vojtěch ; Janovská, Dagmar ; Papoušková, Ludmila
This methodology defines basic terminology connected to on-farm conservation, presents links to international agreements and legal documents and describes its participants. There are presented possible practices of conservation based on experience in Europe, especially ECPGR, FAO and international projects focused on this topic. It proposes the system of on-farm conservation in the conditions of the Czech Republic as well as possibilities of its integration to international/European networks.
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Apple dormant bud cryopreservation: Methodology
Bilavčík, Alois ; Faltus, Miloš ; Zámečník, Jiří
In the Czech Republic, ex situ and in vitro conservation is used for apple tree germplasm conservation. In relation to the strategy of maintenance of broad genetic potential of apple tree, there is a necessity to preserve genotypes by storage at ultralow temperatures – cryoconservation. Based on broad spectrum of apple cultivars, an optimised dormant bud cryopreservation protocol was developed. The developed cryoprotocol enables to establish a safe backup storage system for ex situ and in vitro collections of apple tree germplasm. The Ministry of Agriculture of the Czech Republic is the user of this methodology and it will utilize it in the framework of “National Programme on Conservation and Utilization of Plant, Animal and Microbial Genetic Resources for Food and Agriculture”.
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Chov české slepice
DRÁB, Zdeněk
The main purpose of the bachelor thesis was to collect information about breeding of Czech Hen and the importance of the genetic resources. The history of the breed was described. Another part of the bachelor thesis was the description of the colour strains of the Czech Hen, other similar breeds, the current state, the utility and the principles of breeding. Possible perspectives in breeding of the Czech Hen, its use in creation of laying type of hybrids and possibility of use in organic breeding are suggested at the end of the thesis.
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Old Czech breeds of hens, their conservation and utilization in organic farming.
NAVRÁTIL, Vojtěch
This thesis deals mainly with the only two typical Czech breeds of hens - Šumavanka and Bohemian Fowl. It gathers information about their history and assesses their characteristics, such as stand-alone behavior, demands of breeding, laying of eggs, food searching, resistance in rough climatic conditions. For these breeds, suitability for organic farming is considered. Furthermore, this study focuses on the significance of ecological farming and genetic resources, and their importance for conservation of natural resources for future generations. It also includes examples and practical experiences and results of breeding of Czech hens.
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